Premium
Cell wall degrading enzymes for silage. 2. Aerobic stability of enzyme‐treated laboratory silages
Author(s) -
SELMEROLSEN I.,
HENDERSON A. R.,
ROBERTSON S.,
McGINN R.
Publication year - 1993
Publication title -
grass and forage science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.716
H-Index - 56
eISSN - 1365-2494
pISSN - 0142-5242
DOI - 10.1111/j.1365-2494.1993.tb01836.x
Subject(s) - silage , lolium perenne , fermentation , cellulase , lolium multiflorum , zoology , enzyme , perennial plant , chemistry , food science , formic acid , agronomy , biology , biochemistry
The effects of two commercial cellulase/hemicellulase enzymes derived from Trichoderma reesei on silage fermentation and aerobic stability were investigated in three laboratory‐scale experiments. In Experiment 1, perennial ryegrass ( Lolium perenne ) was treated with enzyme A at the rates of 0. 0·125, 0·250, 0·500 and 0·750 cm 3 kg ‐1 . In Experiment 2, Italian ryegrass ( Lolium multiflorum ) was treated with the same enzyme at the rates of 0, 0·250 and 0·500 cm 3 kg ‐1 and with 85% formic acid (3·5 cm 3 kg ‐1 ). In Experiment 3, perennial ryegrass was ensiled untreated, with enzyme A (0·250 and 0·500 cm 3 kg ‐1 ) and with 0·200 and 0·400 cm 3 kg ‐1 enzyme B which also contained glucose oxidase. All silages were well preserved. Aerobic deterioration was related to numbers of lactate assimilating yeasts. In Experiment 1, all silages were stable over a 4‐day period. In Experiment 2, enzyme A treatment delayed the temperature rise compared with the untreated control ( P <0·05), whereas the formic acid‐treated silage was stable. In Experiment 3, the temperature rise in the silage treated with the higher level of enzyme B occurred one day later than in the other silages ( P <0·05).