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Cell wall degrading enzymes for silage. 1. The fermentation of enzyme‐treated ryegrass in laboratory silos
Author(s) -
SELMEROLSEN I.,
HENDERSON A. R.,
ROBERTSON S.,
McGINN R.
Publication year - 1993
Publication title -
grass and forage science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.716
H-Index - 56
eISSN - 1365-2494
pISSN - 0142-5242
DOI - 10.1111/j.1365-2494.1993.tb01835.x
Subject(s) - silage , lolium perenne , lolium multiflorum , fermentation , cellulase , chemistry , food science , enzyme , acetic acid , lactic acid , agronomy , trichoderma reesei , perennial plant , zoology , biochemistry , biology , bacteria , genetics
The effects of two commercial cellulase/hemicellulase enzymes derived from Trichoderma reesei on silage fermentation were investigated in three laboratory‐scale experiments. In Experiment 1, perennial ryegrass ( Lolium perenne ) was treated with enzyme A at the rates of 0, 0·125, 0·250, 0·500 and 0·750 cm 3 kg ‐1 . In Experiment 2, Italian ryegrass ( Lolium multiflorum ) was treated with the same enzyme at the rates of 0, 0·250 and 0·500 cm 3 kg ‐1 and with 85% formic acid (3·5 cm 3 kg ‐1 ). In Experiment 3, perennial ryegrass was ensiled untreated, with enzyme A (0·250 and 0·500 cm 3 kg ‐1 ) and with 0·200 and 0·400 cm 3 kg ‐1 enzyme B which also contained glucose oxidase. All silages were well preserved. In general enzyme treatment reduced pH and the contents of ammonia nitrogen, neutral detergent fibre and acid detergent fibre, whereas the contents of water‐soluble carbohydrates, acetic acid, lactic acid and ethanol were increased.