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A system for rapid freezing of pasture samples in the field
Author(s) -
COLEBROOK W. F.,
JAMES K. J.,
HAMILTON W. J.
Publication year - 1984
Publication title -
grass and forage science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.716
H-Index - 56
eISSN - 1365-2494
pISSN - 0142-5242
DOI - 10.1111/j.1365-2494.1984.tb01693.x
Subject(s) - pasture , forage , carbon dioxide , environmental science , agronomy , chemistry , biology , organic chemistry
A system for rapid freezing and containment of pasture and forage samples in the field is described. Core temperatures of pasture samples of 2–5 kg mass can be reduced to below 0°C in a few minutes using liquified carbon dioxide in the ratio of 1 to 2 kg carbon dioxide to 1 kg of green plant material. Changes in the pH of the plant extract were very small ( ca. 0 1 pH unit). The system is especially suitable for use in areas remote from normal laboratory facilities, where rapid freezing of herbage samples is essential.