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PREDICTING ORGANIC‐MATTER DIGESTIBILITY FROM IN VIVO AND IN VITRO DETERMINATIONS OF DRY‐MATTER DIGESTIBILITY
Author(s) -
Mcleod M. N.,
Minson D. J.
Publication year - 1974
Publication title -
grass and forage science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.716
H-Index - 56
eISSN - 1365-2494
pISSN - 0142-5242
DOI - 10.1111/j.1365-2494.1974.tb01222.x
Subject(s) - in vivo , in vitro , organic matter , dry matter , zoology , chemistry , biology , microbiology and biotechnology , biochemistry , organic chemistry
The DM digestibility (DMD) and organic matter digestibility (OMD) of 36 tropical grass samples were determined both in vivo and in vitro . Regressions relating OMD to DMD were calculated. In vivo OMD=1·02 in vivo DMD+ 0·1, r=0·99. In vitro OMD=1·05 in vitro DMD—4·7, r=0.99. At the same DMD, the in vitro method predicted OMD 2·8 to 3·4 percentage units lower than the in vivo method. This difference was caused by the difference in mean apparent digestibility of the ash, in vitro 71%, in vivo 47%. In vivo DMD was correlated (r=0·92, P<0·01) with in vitro DMD and the regression relating these two factors had a residual standard deviation of ±1·42 digestibility units. This deviation was reduced to ±1·9 digestibility units when OMD was determined. For both DMD and OMD significant differences were found between grass species in the relation between in vivo and in vitro digestibility. To overcome biased estimates of OMD it was suggested that conversion equations should be avoided, and that the in vitro method be standardized with samples of known in vivo OMD as similar as possible to those being tested.