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A TWO‐STAGE TECHNIQUE FOR THE IN VITRO DIGESTION OF FORAGE CROPS
Author(s) -
Tilley J. M. A.,
Terry R. A.
Publication year - 1963
Publication title -
grass and forage science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.716
H-Index - 56
eISSN - 1365-2494
pISSN - 0142-5242
DOI - 10.1111/j.1365-2494.1963.tb00335.x
Subject(s) - dry matter , forage , rumen , digestion (alchemy) , incubation , agronomy , organic matter , zoology , biology , in vitro , pepsin , chemistry , food science , biochemistry , chromatography , fermentation , enzyme , ecology
A simple technique for the determination in vitro of the dry‐ or organic‐matter digestibility of small (0·5 g) samples of dried forages is described. It involves incubation first with rumen liquor and then with acid pepsin. Using 146 samples of grass, clover and lucerne of known in vivo digestibility ( Y ), the regression equation Y = 0·99 X– 1·01 (S.E. ± 2·31) has been calculated, where X = in vitro dry‐matter digestibility. This technique has been used for the study of the digestibilities of plant breeder's material, of the leaf and stem fractions of herbage and of herbage consumed by animals.