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Changes in CDKN 2 D , TP 53 , and mi R 125a expression: potential role in the evaluation of human amniotic fluid‐derived mesenchymal stromal cell fitness
Author(s) -
MirandaSayago Jose M.,
FernandezArcas Nieves,
ReyesEngel Armando,
Benito Carmen,
Narbona Isidoro,
Alonso Antonio
Publication year - 2012
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2012.01623.x
Subject(s) - biology , microbiology and biotechnology , mesenchymal stem cell , regenerative medicine , embryonic stem cell , microrna , stem cell , gene , genetics
Human amniotic fluid‐derived mesenchymal stromal cells (h AMSC ) have become one of the main cell populations used in regenerative medicine and for the study of various clinical disorders. These cells have a great capacity for proliferation and differentiation and do not form teratomas when transplanted into animal models, and their stemness seems to be between embryonic cells and adult mesenchymal cells. Before their use in cell therapy, they must be cultured and expanded in vitro , but the effect this process has on their fitness, a determining factor for the success or failure of cell therapy, is unknown. We undertook a follow‐up of gene and micro RNA s (mi RNA s) expression using microarray of h AMSC for the first 15 passages. Significant changes were noted in the expression of various m RNA s and mi RNA s, particularly down‐regulation of TP 53 , increased expression of hsa‐mi R ‐125a and up‐regulation of CDKN 2 D . The variations in TP 53 and hsa‐mi R ‐125a may act as an indicator of the stemness of the h AMSC , whereas CDKN 2 D may indicate the begging of early senescence process in a p53‐independent mechanism. The genes described in this study will help evaluate the fitness of h AMSC , thus guaranteeing their biological quality for use in regenerative medicine.