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RNA plasticity and selectivity applicable to therapeutics and novel biosensor development
Author(s) -
Nakamura Yoshikazu,
Ishiguro Akira,
Miyakawa Shin
Publication year - 2012
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2012.01596.x
Subject(s) - aptamer , nucleic acid , biology , oligonucleotide , rna , computational biology , nucleic acid structure , systematic evolution of ligands by exponential enrichment , biochemistry , microbiology and biotechnology , dna , gene
Aptamers are short, single‐stranded nucleic acid sequences that are selected in vitro from large oligonucleotide libraries based on their high affinity to a target molecule. Hence, aptamers can be thought of as a nucleic acid analog to antibodies. However, several viewpoints hold that the potential of aptamers arises from interesting characteristics that are distinct from, or in some cases, superior to those of antibodies. This review summarizes the recent achievements in aptamer programs developed in our laboratory against basic and therapeutic protein targets. Through these studies, we became aware of the remarkable conformational plasticity and selectivity of RNA, on which the published report has not shed much light even though this is evidently a crucial feature for the strong specificity and affinity of RNA aptamers.