Premium
Localized expression of human BMP‐7 by BM‐MSCs enhances renal repair in an in vivo model of ischemia–reperfusion injury
Author(s) -
ZhenQiang Fang,
BingWei Ye,
YongLiang Liu,
XiangWei Wang,
ShanHong Yi,
YuanNing Zhang,
WeiSheng Jia,
Wei Chen,
Ye Gang
Publication year - 2012
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2011.01572.x
Subject(s) - mesenchymal stem cell , biology , in vivo , green fluorescent protein , bone marrow , transplantation , kidney , renal ischemia , malondialdehyde , microbiology and biotechnology , immunology , reperfusion injury , ischemia , endocrinology , medicine , oxidative stress , biochemistry , gene
Ischemia and subsequent reperfusion (I/R) damage kidney tubular cells and consequently impair renal function. Rabbit bone marrow mesenchymal stem cells (BM‐MSCs) expressing human bone morphogenic protein‐7 (hBMP‐7) regenerated tubular cells and improved renal function in a kidney I/R model. Rabbits were injected immediately after I/R with one of the following: (i) hBMP‐7‐transduced BM‐MSCs (BM‐MSCs hBMP‐7 ); (ii) enhanced green fluorescent protein–transduced BM‐MSCs (BM‐MSCs EGFP ); or (iii) PBS. The activity of superoxide dismutase (SOD) was higher, and the amount of malondialdehyde (MDA) was lower in the BM‐MSCs hBMP‐7 group than in the BM‐MSCs EGFP group. Both the BM‐MSCs hBMP‐7 group and the BM‐MSCs EGFP group had higher SOD activity and lower amounts of MDA than the PBS group. Bcl‐2‐ and Bcl‐2‐associated X protein levels, and other variables, indicated the regeneration of the kidney in both experimental groups. However, the BM‐MSCs hBMP‐7 group showed higher activity than the BM‐MSCs EGFP group, indicating that the combined strategy of BM‐MSC transplantation with hBMP‐7 gene therapy could be a useful approach for the treatment of renal IRI.