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Erythropoiesis is regulated by the transcription elongation factor Foggy/Spt5 through gata1 gene regulation
Author(s) -
Taneda Takuya,
Zhu Wenyan,
Cao Qingfu,
Watanabe Hajime,
Yamaguchi Yuki,
Handa Hiroshi,
Wada Tadashi
Publication year - 2011
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2010.01481.x
Subject(s) - gata1 , biology , gene knockdown , transcription factor , erythropoiesis , gata2 , gene , regulation of gene expression , transcription (linguistics) , zebrafish , gene expression , morpholino , haematopoiesis , microbiology and biotechnology , genetics , stem cell , medicine , linguistics , philosophy , anemia
Recent studies have showed that transcription elongation factors regulate early development. Foggy/Spt5 is a subunit of DRB sensitivity‐inducing factor, which negatively and positively regulates transcription elongation. Here, we report that the positive function of Foggy/Spt5 is required for gata1 expression during zebrafish embryonic hematopoiesis. Antisense morpholino oligonucleotide (MO)‐mediated knockdown of foggy/spt5 has led to a reduction in the expression of gata1 and the gata1 target genes alas2 and hbae3 and inhibited proper hemoglobin production. By contrast, expression of hematopoietic stem cell and endothelial markers, including scl , lmo2 , gata2 , fli‐1 , and flk‐1 , and expression of biklf , whose product directs gata1 expression via its direct binding to the gata1 promoter, were unaltered, suggesting that gata1 is a functionally important target gene of Foggy/Spt5. The MO‐mediated gata1 repression was relieved by forced expression of wild‐type foggy/spt5 , but not by a mutant lacking the positive function. Therefore, this study provides evidence that Foggy/Spt5 plays an important role in gata1 gene expression and erythropoiesis through its transcriptional activation domain.