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Necl‐5/PVR enhances PDGF‐induced attraction of growing microtubules to the plasma membrane of the leading edge of moving NIH3T3 cells
Author(s) -
Minami Akihiro,
Mizutani Kiyohito,
Waseda Masazumi,
Kajita Mihoko,
Miyata Muneaki,
Ikeda Wataru,
Takai Yoshimi
Publication year - 2010
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2010.01450.x
Subject(s) - biology , microbiology and biotechnology , dynein , endocytosis , microtubule , leading edge , platelet derived growth factor receptor , protein subunit , integrin , cell membrane , dynactin , biophysics , receptor , membrane , growth factor , biochemistry , materials science , gene , composite material
Microtubules (MTs) search for and grow toward the leading edge of moving cells, followed by their stabilization at a specific structure at the rear site of the leading edge. This dynamic re‐orientation of MTs is critical to directional cell movement. We previously showed that Necl‐5/poliovirus receptor (PVR) interacts with platelet‐derived growth factor (PDGF) receptor and integrin α v β 3 at the leading edge of moving NIH3T3 cells, resulting in an enhancement of their directional movement. We studied here the role of Necl‐5 in the PDGF‐induced attraction of growing MTs to the leading edge of NIH3T3 cells. Necl‐5 enhanced the PDGF‐induced growth of MTs and attracted them near to the plasma membrane of the leading edge of NIH3T3 cells in an integrin α v β 3 ‐dependent manner. Furthermore, Necl‐5 enhanced the PDGF‐induced attraction of the plus‐end‐tracking proteins (+TIPs), including EB1, CLIP170, an intermediate chain subunit of cytoplasmic dynein, and p150 Glued , a subunit of dynactin, near to the plasma membrane of the leading edge. Thus, Necl‐5 plays a role in the attraction of growing MTs to the plasma membrane of the leading edge of moving cells.