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Chromatin remodeling protein INO80 has a role in regulation of homeotic gene expression in Drosophila
Author(s) -
Bhatia Shipra,
Pawar Hema,
Dasari Vasanthi,
Mishra Rakesh K.,
Chandrashekaran Shanti,
Brahmachari Vani
Publication year - 2010
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2010.01416.x
Subject(s) - homeotic gene , ultrabithorax , biology , antennapedia , hox gene , genetics , caenorhabditis elegans , drosophila melanogaster , gene , mutant , krüppel , chromatin , p element , regulation of gene expression , microbiology and biotechnology , drosophilidae , gene expression
The homologues of yeast INO80 are identified across phyla from Caenorhabditis elegans to human. In Drosophila it has been shown that dINO80 forms a complex with Pleiohomeotic but does not interact with Hox PRE (polycomb responsive element). As some proteins of the INO80 complex are implicated in homeotic gene regulation, we examined if dINO80 is involved in regulation of homeotic genes. We find that dINO80 null mutants generated by imprecise excision of P‐element are late embryonic lethals and show homeotic transformation. We detect misexpression of homeotic genes like Sex‐comb reduced , Antennapedia , Ultrabithorax and Abdominal‐B in dIno80 mutant embryos by immunostaining which is further substantiated by quantitative PCR. Polycomb phenotype in dIno80‐Pc is enhanced in double mutants. Concurrently, the localization of dINO80 to sequences upstream of misexpressed genes in vivo shows that dINO80 is involved in homeotic gene regulation and probably through its interactions with PcG‐trxG complexes.