Premium
Novel role for the intraflagellar transport protein CMG‐1 in regulating the transcription of cyclin‐D2 , E‐cadherin and integrin‐α family genes in mouse spermatocyte‐derived cells
Author(s) -
ichiro Ohbayashi Ken,
Tanaka Kiyoko,
Kitajima Kenji,
Tamura Kazuhiro,
Hara Takahiko
Publication year - 2010
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2010.01414.x
Subject(s) - biology , gene knockdown , microbiology and biotechnology , enhancer , gene , gene expression , genetics
Capillary morphogenesis gene (CMG)‐1 is a mammalian homologue of the intraflagellar transport protein IFT‐74/72 of Chlamydomonas . CMG‐1 is abundantly expressed in immature stages of male germ‐line cells of the adult mouse testis and is required for the expression of cyclin‐D2 in GC‐2, a mouse premeiotic spermatocyte‐derived cell line. In this study, we show that the knockdown of CMG‐1 in GC‐2 cells leads to down‐regulation of E‐cadherin , integrin‐α1 , α2 , α10 , and α11 expression. The ability of the CMG‐1 ‐knockdown GC‐2 cells to adhere to type‐I collagen‐coated plates was consequently impaired. Inducible expression of an siRNA‐resistant CMG‐1 cDNA in these cells rescued the expression of E‐cadherin and the integrin‐α family genes and partially restored adherence to type‐I collagen. CMG‐1 participates in the transcriptional regulation of cyclin‐D2 via a genomic DNA region between −250 and −216 of the mouse cyclin‐D2 gene. Closely related sequences were found in the enhancer/promotor regions of E‐cadherin and the four integrin‐α family genes. Based on these data, we propose that CMG‐1 serves as a transcriptional regulator of proliferation and adhesion‐associated genes in early stage male germ‐line cells in the testis.