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Novel RNA polymerase II mutation suppresses transcriptional fidelity and oxidative stress sensitivity in rpb9Δ yeast
Author(s) -
Koyama Hiroshi,
Ueda Tomofumi,
Ito Takahiro,
Sekimizu Kazuhisa
Publication year - 2010
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2009.01372.x
Subject(s) - biology , rna polymerase ii , transcription (linguistics) , rna polymerase , transcription factor , microbiology and biotechnology , rna , gene expression , gene , genetics , promoter , linguistics , philosophy
We previously reported that transcription elongation factor S‐II and RNA polymerase II subunit Rpb9 maintain transcriptional fidelity and contribute to oxidative stress resistance in yeast. Here we examined whether other transcription elongation‐related factors affect transcriptional fidelity in vivo . Among the 17 mutants of transcription elongation‐related factors analyzed, most were not responsible for maintaining transcriptional fidelity. This finding indicates that transcriptional fidelity is controlled by a limited number of transcription elongation‐related factors including S‐II and Rpb9 and not by all transcription elongation‐related factors. In contrast, by screening rpb9Δ cell revertants for sensitivity to the oxidant menadione, we identified a novel mutation in RNA polymerase II, rpb1‐G730D , which suppressed both reduced transcriptional fidelity and oxidative stress sensitivity. These findings suggest that the maintenance of transcriptional fidelity that is mediated by transcription machinery directly confers oxidative stress resistance.