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Regulatory mechanism for expression of GPX1 in response to glucose starvation and Ca 2+ in Saccharomyces cerevisiae : involvement of Snf1 and Ras/cAMP pathway in Ca 2+ signaling
Author(s) -
Ohdate Takumi,
Izawa Shingo,
Kita Keiko,
Inoue Yoshiharu
Publication year - 2010
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2009.01365.x
Subject(s) - gpx1 , biology , gpx3 , kinase , protein kinase a , biochemistry , gpx4 , glutathione , microbiology and biotechnology , enzyme , glutathione peroxidase
Saccharomyces cerevisiae has three homologues of the glutathione peroxidase gene, GPX1 , GPX2 , and GPX3 . We have previously reported that the expression of GPX3 was constitutive, but that of GPX2 was induced by oxidative stress and CaCl 2 , and uncovered the regulatory mechanisms involved. Here, we show that the expression of GPX1 is induced by glucose starvation and treatment with CaCl 2 . The induction of GPX1 expression in response to glucose starvation and Ca 2+ was dependent on the transcription factors Msn2 and Msn4 and cis ‐acting elements [stress response element (STRE)] in the GPX1 promoter. The Ras/cAMP pathway is also involved in the expression of GPX1 . We found that Snf1, a Ser/Thr protein kinase, is involved in the glucose starvation‐ and Ca 2+ ‐induced expression of GPX1. The activation of Snf1 is accompanied by phosphorylation of Thr 210 . We found that the Ca 2+ ‐treatment as well as glucose starvation causes the phosphorylation of Thr 210 of Snf1 in a Tos3, Sak1, and Elm1 protein kinase‐dependent manner. As the timing of the initiation of Ca 2+ ‐induced expression of GPX1 was retarded in an snf1 Δ mutant, the activation of Snf1 seems pivotal to the early‐stage‐response of GPX1 to Ca 2+ .