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Functional analysis of GS28, an intra‐Golgi SNARE, in Caenorhabditis elegans
Author(s) -
Maekawa Masashi,
Inoue Takao,
Kobuna Hiroyuki,
Nishimura Taki,
GengyoAndo Keiko,
Mitani Shohei,
Arai Hiroyuki
Publication year - 2009
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2009.01325.x
Subject(s) - golgi apparatus , biology , microbiology and biotechnology , caenorhabditis elegans , mutant , gene knockdown , embryonic stem cell , rna interference , phenotype , secretory pathway , genetics , gene , endoplasmic reticulum , rna
Intra‐Golgi retrograde transport is assumed to maintain Golgi function by recycling Golgi‐resident proteins to younger cisternae in the progression of entire Golgi stack from cis to trans . GS28 (Golgi SNARE of 28 kDa, also known as GOS28) is a Golgi‐localized SNARE protein and has been implicated in intra‐Golgi retrograde transport. However, the in vivo functions of GS28, and consequently, the roles of the intra‐Golgi retrograde transport in animal development are largely unknown. In this study, we generated deletion mutants of Caenorhabditis elegans GS28 and performed a synthetic lethal RNAi screen using GS28 mutants. We found that another Golgi‐localized SNARE, Ykt6, functions cooperatively with GS28 in embryonic development. During post‐embryonic development, GS28 mutants exhibited reduced seam cell numbers and a missing ray phenotype under Ykt6 knockdown conditions, suggesting that cell proliferation and/or differentiation of stem cell‐like seam cells are impaired in GS28‐ and Ykt6‐depleted worms. We also demonstrated that GS28 and Ykt6 act redundantly for the proper expression of Golgi‐resident proteins in adult intestinal cells . This study reveals the in vivo importance of the Golgi‐localized SNAREs GS28 and Ykt6.