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Ctf4 coordinates the progression of helicase and DNA polymerase α
Author(s) -
Tanaka Hirokazu,
Katou Yuki,
Yagura Masaru,
Saitoh Katsuya,
Itoh Takehiko,
Araki Hiroyuki,
Bando Masashige,
Shirahige Katsuhiko
Publication year - 2009
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2009.01310.x
Subject(s) - replisome , biology , helicase , primase , dna replication , dna polymerase , dna polymerase ii , establishment of sister chromatid cohesion , control of chromosome duplication , microbiology and biotechnology , genetics , dna polymerase delta , eukaryotic dna replication , dna , gene , polymerase chain reaction , chromatin , reverse transcriptase , rna , cohesin
Ctf4 is a protein conserved in eukaryotes and a constituent of the replisome progression complex. It also plays a role in the establishment of sister chromatid cohesion. In our current study, we demonstrate that the replication checkpoint is activated in the absence of Ctf4, and that the interaction between the MCM helicase‐go ichi ni san (GINS) complex and DNA polymerase α (Pol α)‐primase is destabilized specifically in a ctf4Δ mutant. An in vitro interaction between GINS and DNA Pol α was also found to be mediated by Ctf4. The same interaction was not affected in the absence of the replication checkpoint mediators Tof1 or Mrc1. In ctf4Δ cells, DNA pol α became significantly unstable and was barely detectable at the replication forks in HU. In contrast, the quantities of helicase and DNA pol ɛ bound to replication forks were almost unchanged but their localizations were widely and abnormally dispersed in the mutant cells compared with wild type. These results lead us to propose that Ctf4 is a key connector between DNA helicase and Pol α and is required for the coordinated progression of the replisome.