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SAP‐1 is a microvillus‐specific protein tyrosine phosphatase that modulates intestinal tumorigenesis
Author(s) -
Sadakata Hisanobu,
Okazawa Hideki,
Sato Takashi,
Supriatna Yana,
Ohnishi Hiroshi,
Kusakari Shinya,
Murata Yoji,
Ito Tomokazu,
Nishiyama Uichi,
Minegishi Takashi,
Harada Akihiro,
Matozaki Takashi
Publication year - 2009
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2008.01270.x
Subject(s) - biology , carcinogenesis , protein tyrosine phosphatase , microvillus , brush border , adenomatous polyposis coli , intestinal epithelium , microbiology and biotechnology , tyrosine , fibronectin , epithelium , phosphorylation , gene , biochemistry , extracellular matrix , genetics , cancer , colorectal cancer , vesicle , membrane
SAP‐1 (PTPRH) is a receptor‐type protein tyrosine phosphatase (RPTP) with a single catalytic domain in its cytoplasmic region and fibronectin type III‐like domains in its extracellular region. The cellular localization and biological functions of this RPTP have remained unknown, however. We now show that mouse SAP‐1 mRNA is largely restricted to the gastrointestinal tract and that SAP‐1 protein localizes to the microvilli of the brush border in gastrointestinal epithelial cells. The expression of SAP‐1 in mouse intestine is minimal during embryonic development but increases markedly after birth. SAP‐1‐deficient mice manifested no marked changes in morphology of the intestinal epithelium. In contrast, SAP‐1 ablation inhibited tumorigenesis in mice with a heterozygous mutation of the adenomatous polyposis coli gene. These results thus suggest that SAP‐1 is a microvillus‐specific RPTP that regulates intestinal tumorigenesis.