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Effects of Akt signaling on nuclear reprogramming
Author(s) -
Nakamura Toshinobu,
Inoue Kimiko,
Ogawa Shoko,
Umehara Hiroki,
Ogonuki Narumi,
Miki Hiromi,
Kimura Tohru,
Ogura Atsuo,
Nakano Toru
Publication year - 2008
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2008.01243.x
Subject(s) - reprogramming , biology , microbiology and biotechnology , somatic cell , protein kinase b , embryonic stem cell , cell potency , cellular differentiation , induced pluripotent stem cell , somatic cell nuclear transfer , cell fusion , stem cell , induced stem cells , signal transduction , cell , embryo , genetics , embryogenesis , blastocyst , gene
Reprogramming of the epigenetic state from differentiated to pluripotent cells can be attained by cell fusion of differentiated somatic cells with embryonic stem (ES) cells or transfer of the nucleus of a differentiated cell into an enucleated oocyte. Activation of Akt signaling is sufficient to maintain pluripotency of ES cells and promotes derivation of embryonic germ (EG) cells from primordial germ cells (PGCs). Here we analyzed the effects of Akt signaling on somatic cell nuclear reprogramming after cell fusion and nuclear transfer. We found that forced activation of Akt signaling stimulated reprogramming after cell fusion of ES cells with thymocytes or mouse embryonic fibroblasts. These hybrid cells showed ES cell characteristics, including in vitro and in vivo differentiation capacity. In contrast, Akt signaling significantly reduced the efficiency of reprogramming with nuclear transfer. Our results demonstrate that Akt signaling plays important roles on the nuclear reprogramming of somatic cells.