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Nucleoredoxin regulates the Wnt/planar cell polarity pathway in Xenopus
Author(s) -
Funato Yosuke,
Michiue Tatsuo,
Terabayashi Takeshi,
Yukita Akira,
Danno Hiroki,
Asashima Makoto,
Miki Hiroaki
Publication year - 2008
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2008.01220.x
Subject(s) - dishevelled , xenopus , wnt signaling pathway , morpholino , convergent extension , gastrulation , biology , microbiology and biotechnology , cell polarity , zebrafish , phenotype , signal transduction , frizzled , cell , genetics , gene , embryo , embryogenesis
The Wnt signaling pathway is conserved across species, and is essential for early development. We previously identified nucleoredoxin (NRX) as a protein that interacts with dishevelled (Dvl) in vivo to negatively regulate the Wnt/β‐catenin pathway. However, whether NRX affects another branch of the Wnt pathway, the Wnt/planar cell polarity (PCP) pathway, remains unclear. Here we show that NRX regulates the Wnt/PCP pathway. In Xenopus laevis , over‐expression or depletion of NRX by injection of NRX mRNA or antisense morpholino oligonucleotide, respectively, yields the bent‐axis phenotype that is typically observed in embryos with abnormal PCP pathway activity. In co‐injection experiments of Dvl and NRX mRNA, NRX suppresses the Dvl‐induced bent‐axis phenotype. Over‐expression or depletion of NRX also suppresses the convergent extension movements that are believed to underlie normal gastrulation. We also found that NRX can inhibit Dvl‐induced up‐regulation of c‐Jun phosphorylation. These results indicate that NRX plays crucial roles in the Wnt/PCP pathway through Dvl and regulates Xenopus gastrulation movements.