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Cop9 signalosome subunit 8 (CSN8) is essential for Drosophila development
Author(s) -
OrenGiladi Pazit,
Krieger Ofra,
Edgar Bruce A.,
Chamovitz Daniel A.,
Segal Daniel
Publication year - 2008
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2008.01164.x
Subject(s) - biology , cop9 signalosome , mutant , protein subunit , phenotype , mitosis , microbiology and biotechnology , regulator , genetics , drosophila (subgenus) , gene , biochemistry , protease , peptide hydrolases , enzyme
The COP9 signalosome (CSN) is a multisubunit regulator highly conserved in evolution. We show here that CSN subunit 8 (CSN8) is essential for Drosophila development. CSN8 is maternally contributed and present throughout development. Null mutants generated in this study are larval lethal, showing phenotypes associated with mutations in either CSN4 (molting defects) or CSN5 (melanotic tumors). Analysis of mitotic and germ‐line csn8 null clones revealed the requirement of CSN8 for multiple developmental processes. The germ‐line clones arrested at mid‐oogenesis, while the mitotic clones led to deformed adult eyes or wings. CSN8 is present exclusively as part of the CSN holo‐complex, and lack of CSN8 in the mutants leads to CSN instability. Consistent with this, Cullin deneddylation is impaired in the csn8 null mutants.