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Identification of ZNF200 as a novel binding partner of histone H3 methyltransferase G9a
Author(s) -
Nishida Miki,
Kato Masaki,
Kato Yasuko,
Sasai Nobuhiro,
Ueda Jun,
Tachibana Makoto,
Shinkai Yoichi,
Yamaguchi Masamitsu
Publication year - 2007
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2007.01098.x
Subject(s) - biology , zinc finger , histone methyltransferase , methyltransferase , histone h3 , subfamily , amino acid , genetics , immunoprecipitation , phd finger , complementary dna , peptide sequence , sequence alignment , homology (biology) , microbiology and biotechnology , biochemistry , methylation , lysine , gene , transcription factor
G9a belongs to the subfamily of histone H3 lysine 9 (H3‐K9)‐specific methyltransferases. On amino acid sequence alignment of human and Drosophila G9a, we found that the N‐terminal region from amino acids 532–605 to be evolutionarily conserved and named this the G9a homology domain (GHD). Using the GHD of human G9a (hG9a) as a bait, we isolated cDNA encoding a zinc finger protein 200 (ZNF200), which contains five C 2 H 2 ‐type zinc finger domains in tandem arrays. Interaction between G9a and ZNF200 could be demonstrated by in vitro binding assays and immunoprecipitation experiments using cultured human HEK293 cell extracts. GST pull‐down assays using deletion derivatives of ZNF200 revealed that the interaction is through a region encompassing three of the five zinc finger domains. Furthermore, ZNF200 appear to co‐localize with G9a in the nucleoplasm of HEK293 cells as discrete speckles. These results demonstrate that ZNF200 is a novel binding partner of G9a.