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Dynamics of yeast prion aggregates in single living cells
Author(s) -
KawaiNoma Shigeko,
Ayano Satoru,
Pack ChanGi,
Kinjo Masataka,
Yoshida Masasuke,
Yasuda Kenji,
Taguchi Hideki
Publication year - 2006
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2006.01004.x
Subject(s) - biology , yeast , phenotype , cytoplasm , microbiology and biotechnology , fluorescence correlation spectroscopy , prion protein , protein aggregation , cell , oligomer , biophysics , fluorescence , green fluorescent protein , in vivo , biochemistry , genetics , chemistry , gene , medicine , physics , disease , organic chemistry , pathology , quantum mechanics
Prions are propagating proteins that are ordered protein aggregates, in which the phenotypic trait is retained in the altered protein conformers. To understand the dynamics of the prion aggregates in living cells, we directly monitored the fate of the aggregates using an on‐chip single‐cell cultivation system as well as fluorescence correlation spectroscopy (FCS). Single‐cell imaging revealed that the visible foci of yeast prion Sup35 fused with GFP are dispersed throughout the cytoplasm during cell growth, but retain the prion phenotype. FCS showed that [PSI + ] cells, irrespective of the presence of foci, contain diffuse oligomers, which are transmitted to their daughter cells. Single‐cell observations of the oligomer‐based transmission provide a link between previous in vivo and in vitro analyses of the prion and shed light on the relationship between the protein conformation and the phenotype.