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Over‐expression of c‐Myb increases the frequency of hemogenic precursors in the endothelial cell population
Author(s) -
Dai Guoyou,
Sakamoto Hiroshi,
Shimoda Yuri,
Fujimoto Tetsuhiro,
Nishikawa ShinIchi,
Ogawa Minetaro
Publication year - 2006
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2006.00985.x
Subject(s) - biology , myb , microbiology and biotechnology , population , expression (computer science) , genetics , transcription factor , gene , computer science , programming language , demography , sociology
Definitive hematopoiesis has been proposed to arise from hemogenic endothelial cells during mouse embryogenesis. The c‐ myb proto‐oncogene is essential for the development of definitive hematopoiesis and was reported to be activated in hemogenic endothelial cells. To investigate whether c‐Myb is involved in regulating the development of hemogenic endothelial cells, we conditionally induced c‐ myb over‐expression during the in vitro differentiation of embryonic stem cells. VE‐cadherin + CD45 − cells inducibly expressing c‐Myb showed an increase in multilineage colony formation as well as an augmented capacity of the colony forming cells to self‐renew in vitro under the condition that only the endogenous c‐ myb gene was expressed during differentiation of hematopoietic cells. Over‐expression of c‐Myb in the endothelial population led to activation of genes associated with definitive hematopoiesis such as Runx1 , Hoxb4 , Mll and Etv6 . Our data provide evidence that c‐Myb is able to exert an effect in endothelial cells which fosters the establishment of their hemogenic potential.