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Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP‐A chromatin of human cells
Author(s) -
Izuta Hiroshi,
Ikeno Masashi,
Suzuki Nobutaka,
Tomonaga Takeshi,
Nozaki Naohito,
Obuse Chikashi,
Kisu Yasutomo,
Goshima Naoki,
Nomura Fumio,
Nomura Nobuo,
Yoda Kinya
Publication year - 2006
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2006.00969.x
Subject(s) - centromere , kinetochore , biology , sister chromatids , chromatin , interphase , microbiology and biotechnology , metaphase , mitosis , chromosome segregation , aurora b kinase , human artificial chromosome , chromosome , genetics , dna , gene
The centromere is a chromatin structure essential for correct segregation of sister chromatids, and defects in this region often lead to aneuploidy and cancer. We have previously reported purification of the interphase centromere complex (ICEN) from HeLa cells, and have demonstrated the presence of 40 proteins (ICEN1–40), along with CENP‐A, ‐B, ‐C, ‐H and hMis6, by proteomic analysis. Here we report analysis of seven ICEN components with unknown function. Centromere localization of EGFP‐tagged ICEN22, 24, 32, 33, 36, 37 and 39 was observed in transformant cells. Depletion of each of these proteins by short RNA interference produced abnormal metaphase cells carrying misaligned chromosomes and also produced cells containing aneuploid chromosomes, implying that these ICEN proteins take part in kinetochore functions. Interestingly, in the ICEN22, 32, 33, 37 or 39 siRNA‐transfected cells, CENP‐H and hMis6 signals disappeared from all the centromeres in abnormal mitotic cells containing misaligned chromosomes. These results suggest that the seven components of the ICEN complex are predominantly localized at the centromeres and are required for kinetochore function perhaps through or not through loading of CENP‐H and hMis6 onto the centromere.