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Does mPER2 protein oscillate without its coding mRNA cycling?: post‐transcriptional regulation by cell clock
Author(s) -
Fujimoto Yoshito,
Yagita Kazuhiro,
Okamura Hitoshi
Publication year - 2006
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2006.00960.x
Subject(s) - biology , messenger rna , microbiology and biotechnology , coding region , gene , genetics
Does the mammalian oscillatory protein mPER2 show the rhythm without its coding mRNA cycling? Here we answer this question by inserting a single copy of exogenous mPer2 gene to a NIH3T3 fibroblasts cell line, using Flp‐In system. We generated the stable cell lines which constantly express mRNAs coding either N‐terminal FLAG‐tagged full length mPER2 (FLAG‐mPER2(full)) or its C‐terminal deleted form (FLAG‐mPER2(1–1068)), which lacks the binding site to mCRY proteins, under the control of human EF‐1α promoter. Although serum shock induced the rhythm of endogenous clock machinery in these cell lines, it did not initiate the rhythm of exogenously inserted FLAG‐mPer2 genes at the mRNA level. In contrast, FLAG‐mPER2(full) proteins showed the rhythm without their coding mRNA cycling. Since cells expressing FLAG‐mPER2(1–1068) also showed the rhythm of FLAG‐mPER2(1–1068) proteins, the direct binding of mCRY and mPER2 seems not necessary for this protein oscillation. This system clearly demonstrates that the intracellular endogenous clock system has an ability to modify the mPer2 gene post‐transcriptionally to make mPER2 proteins oscillate without its coding mRNA cycling.