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Odd‐Skipped Related 2 gene transcription is regulated by CCAAT enhancer‐binding protein δ in mesenchymal C3H10T1/2 cells
Author(s) -
Kawai Shinji,
Kato Takahiro,
Sato Masahiro,
Amano Atsuo
Publication year - 2006
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2006.00929.x
Subject(s) - biology , chromatin immunoprecipitation , enhancer , microbiology and biotechnology , gene , mutant , binding site , transcription factor , electrophoretic mobility shift assay , repressor , phenotype , regulation of gene expression , promoter , genetics , gene expression
Odd‐skipped related 2 ( Osr2 ) gene is mouse homolog of Drosophila Odd‐skipped gene involved with the pair‐rule segmentation phenotype in Drosophila mutant embryos. In this study, to examine Osr2 expression regulation, the mouse Osr2 promoter region was cloned and characterized, and found to have two enhancer elements in the −1463/−1031 (distal) and −581/+3 (proximal) regions, and a repressor region (−4845/−1463, far distal). CCAAT/enhancer binding protein ( C/EBP ) binding sites were found in both the distal and proximal enhancer elements. Osr2 promoter activity was enhanced by C/EBPδ , a member of the C/EBP family, in a dose‐dependent manner. Electrophoresis mobility shift assays showed that purified GST‐C/EBPδ bound to distal (−1295/−1261) and proximal (−89/−55) C/EBP binding motifs. Chromatin immunoprecipitation demonstrated that acetylated histones H3, H4, and C/EBPδ in the proximal region (−280/−43), but not the distal region (−1438/−1196), indicating that the Osr2 promoter proximal region was transcriptionally activated in C3H10T1/2 cells. Our results suggest that Osr2 expression is regulated by C/EBP regulatory elements.