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Pax6 is required for production and maintenance of progenitor cells in postnatal hippocampal neurogenesis
Author(s) -
Maekawa Motoko,
Takashima Noriko,
Arai Yoko,
Nomura Tadashi,
Inokuchi Kaoru,
Yuasa Shigeki,
Osumi Noriko
Publication year - 2005
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2005.00893.x
Subject(s) - neurogenesis , pax6 , subgranular zone , biology , progenitor cell , hippocampal formation , dentate gyrus , progenitor , microbiology and biotechnology , embryonic stem cell , stem cell , neuroscience , transcription factor , genetics , subventricular zone , gene
Neurogenesis is crucial for brain formation and continues to take place in certain regions of the postnatal brain including the subgranular zone (SGZ) of the hippocampal dentate gyrus (DG). Pax6 transcription factor is a key player for patterning the brain and promoting embryonic neurogenesis, and is also expressed in the SGZ. In the DG of wild‐type rats, more than 90% of total BrdU‐incorporated cells expressed Pax6 at 30 min time point after BrdU injection. Moreover, approximately 60% of Pax6 + cells in the SGZ exhibited as GFAP + cells with a radial glial phenotype and about 30% of Pax6 + cells exhibited as PSA‐NCAM + cells in clusters. From BrdU labeling for 3 days, we found that cell proliferation was 30% decreased at postnatal stages in Pax6 ‐deficient rSey 2 /+ rat. BrdU pulse/chase experiments combined with marker staining revealed that PSA‐NCAM + late progenitor cells increased at the expense of GFAP + early progenitors in rSey 2 /+ rat. Furthermore, expression of Wnt ligands in the SGZ was markedly reduced in rSey 2 /+ rat. Taken all together, an appropriate dosage of Pax6 is essential for production and maintenance of the GFAP + early progenitor cells in the postnatal hippocampal neurogenesis.