z-logo
Premium
Recruitment of E‐cadherin associated with α‐ and β‐catenins and p120 ctn to the nectin‐based cell‐cell adhesion sites by the action of 12‐O‐tetradecanoylphorbol‐13‐acetate in MDCK cells
Author(s) -
Okamoto Ryoko,
Irie Kenji,
Yamada Akio,
Katata Tatsuo,
Fukuhara Atsunori,
Takai Yoshimi
Publication year - 2005
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2005.00846.x
Subject(s) - cadherin , nectin , adherens junction , catenin , cell adhesion molecule , microbiology and biotechnology , occludin , biology , cell adhesion , tight junction , adhesion , cell junction , cell , chemistry , biochemistry , signal transduction , wnt signaling pathway , organic chemistry
The formation of tight junctions (TJs) is dependent on the formation of adherens junctions (AJs) in MDCK cells. E‐Cadherin and nectin are major cell‐cell adhesion molecules (CAMs) at AJs, whereas claudin, occludin and junctional adhesion molecule (JAM) are major CAMs at TJs. When MDCK cells precultured at 2 µ m Ca 2+ are cultured at 2 m m Ca 2+ , nectin first forms cell‐cell adhesion and recruits E‐cadherin to the nectin‐based cell‐cell adhesion sites to form AJs. Thereafter, nectin recruits first JAM‐A and then claudin‐1 and occludin to the apical side of AJs to form TJs. In contrast, when MDCK cells precultured at 2 µ m Ca 2+ are cultured at 2 µ m Ca 2+ in the presence of a phorbol ester, 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), a TJ‐like structure is formed without the formation of the E‐cadherin‐based AJs. We showed here that GFP‐E‐cadherin, which did not trans ‐interact due to 2 µ m Ca 2+ but associated with α‐ and β‐catenins and p120 ctn , was recruited to the nectin‐based cell‐cell adhesion sites by the action of TPA. The nectin inhibitors, which inhibited the trans ‐interaction of nectin, inhibited the recruitment of GFP‐E‐cadherin and their associating catenins by the action of TPA. Microbeads coated with the extracellular fragment of nectin recruited not only cellular nectin but also GFP‐E‐cadherin and their associating catenins by the action of TPA. These results indicate that when the TJ‐like structure is formed by the action of TPA, non‐ trans ‐interacting E‐cadherin and its associating catenins are recruited to the nectin‐based cell‐cell adhesion sites and that the trans ‐interaction of E‐cadherin is not essential for the formation of TJs.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here