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53BP2 induces apoptosis through the mitochondrial death pathway
Author(s) -
Kobayashi Shinya,
Kajino Shinichi,
Takahashi Naoko,
Kanazawa Satoshi,
Imai Kenichi,
Hibi Yurina,
Ohara Hirotaka,
Itoh Makoto,
Okamoto Takashi
Publication year - 2005
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2005.00835.x
Subject(s) - biology , apoptosis , microbiology and biotechnology , mitochondrion , programmed cell death , genetics
The p53 binding protein 2 (53BP2) has been identified as the interacting protein to p53, Bcl‐2, and p65 subunit of nuclear factor κB (NF‐κB). The TP53BP2 gene encodes two splicing variants, 53BP2S and 53BP2L, previously known as apoptosis stimulating protein 2 of p53 (ASPP2). We found that these 53BP2 proteins are located predominantly in the cytoplasm and induce apoptosis as demonstrated by cleavage of poly ADP ribose polymerase (PARP) and annexin V staining. Furthermore, we demonstrate that 53BP2 is located in the mitochondria and induces apoptosis associated with depression of the mitochondrial trans‐membrane potential (ΔΨm) and activation of caspase‐9. From these findings we conclude that 53BP2 induces apoptosis through the mitochondrial death pathway.