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Tissue expression of four troponin I genes and their molecular interactions with two troponin C isoforms in Caenorhabditis elegans
Author(s) -
Ruksana Razia,
Kuroda Kazuki,
Terami Hiromi,
Bando Tetsuya,
Kitaoka Shun,
Takaya Tomohide,
Sakube Yasuji,
Kagawa Hiroaki
Publication year - 2005
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2005.00829.x
Subject(s) - biology , gene isoform , troponin c , gene knockdown , troponin i , gene , gene duplication , caenorhabditis elegans , rna interference , microbiology and biotechnology , genetics , troponin complex , rna , medicine , myocardial infarction
Gene duplication is a major genetic event that can produce multiple protein isoforms. Comparative sequence and functional analysis of related gene products can provide insights into protein family evolution. To characterize the Caenorhabditis elegans troponin I family, we analyzed gene structures, tissue expression patterns and RNAi phenotypes of four troponin I isoforms. Tissue expression patterns were determined using lacZ/gfp/rfp reporter gene assays. The tni‐1 , tni‐2 / unc‐27 and tni‐3 genes, each encoding a troponin I isoform, are uniquely expressed in body wall, vulval and anal muscles but at different levels; tni‐4 was expressed solely in the pharynx. Expressing tni‐1 and ‐2 gene RNAi caused motility defects similar to unc‐27 ( e155 ) mutant, a tni‐2 null allele. The tni‐3 RNAi expression produced egg laying defects while the tni‐4 RNAi caused arrest at gastrulation. Overlay analyses were used to assay interactions between the troponin I and two troponin C isoforms. The three body wall troponin I isoforms interacted with body wall and pharyngeal troponin C isoforms; TNI‐4 interacted only with pharyngeal troponin C. Our results suggest the body wall genes have evolved following duplication of the pharynx gene and provide important data about gene duplication and functional differentiation of nematode troponin I isoforms.