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Differentiation status dependent function of FOG‐1
Author(s) -
Tanaka Makoto,
Zheng Jie,
Kitajima Kenji,
Kita Keisuke,
Yoshikawa Hideki,
Nakano Toru
Publication year - 2004
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2004.00796.x
Subject(s) - biology , haematopoiesis , cellular differentiation , microbiology and biotechnology , transcription factor , context (archaeology) , embryonic stem cell , stem cell , gene , genetics , paleontology
The molecular interactions between transcription factors and cofactors play crucial roles in various biological processes, including haematopoiesis. FOG‐1 is a cognate cofactor of GATA‐1, and the FOG‐1/GATA‐1 complex is essential for the haematopoietic differentiation of erythroid cells and megakaryocytes. In order to elucidate the biological functions of FOG‐1 in the different contexts of cell differentiation, we analysed the effects of FOG‐1 expression on haematopoietic cell differentiation, using a combination of in vitro differentiation of mouse embryonic stem (ES) cells and conditional gene expression. FOG‐1 suppressed the proliferation of primitive and definitive erythroid cells in all stages of differentiation. However, FOG‐1 inhibited and enhanced megakaryopoiesis in the early and late differentiation stages, respectively, through different molecular mechanisms. In addition, FOG‐1 inhibited the proliferation of ES cells, the molecular mechanism of which differs from those of erythroid and megakaryocytic cells. These results suggest that FOG‐1 functions in a cell differentiation context‐dependent manner.