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GRIP1τ, a novel PDZ domain‐containing transcriptional activator, cooperates with the testis‐specific transcription elongation factor SII‐T1
Author(s) -
Nakata Akihito,
Ito Takahiro,
Nagata Makiko,
Hori Sayaka,
Sekimizu Kazuhisa
Publication year - 2004
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1365-2443.2004.00795.x
Subject(s) - transactivation , biology , transcription factor , pdz domain , activator (genetics) , dna binding protein , microbiology and biotechnology , dna binding domain , immunoprecipitation , transcription (linguistics) , promoter , receptor , gene , genetics , gene expression , linguistics , philosophy
SII‐T1 is a tissue‐specific member of the transcription elongation factor S‐II that is expressed specifically in male germ cells. In the present study, we have identified a protein named GRIP1τ interacting with SII‐T1 by yeast two‐hybrid screening. GRIP1τ is a novel isoform of glutamate receptor‐interacting protein 1 (GRIP1) that associates with the cytoplasmic domain of the α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoaxazolepropionate (AMPA)‐type glutamate receptor. GRIP1τ is a testis‐specific nuclear protein that activates transcription when fused with a GAL4 DNA binding domain in GAL4‐responsive reporter gene assays. The transactivation domain of GRIP1τ overlapped with the region essential for interaction with SII‐T1, as revealed by co‐immunoprecipitation assays. Also, transactivation by GRIP1τ was stimulated by SII‐T1 in a dose‐dependent manner. Therefore, we propose that GRIP1τ is a novel testis‐specific transcriptional activator regulated by interaction with the testis‐specific transcription elongation factor SII‐T1.