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A deep maximum of green sulphur bacteria (‘ Chlorochromatium aggregatum ’) in a strongly stratified reservoir
Author(s) -
Chapin Bridgett R. K.,
DeNoyelles Frank,
Graham David W.,
Smith Val H.
Publication year - 2004
Publication title -
freshwater biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.297
H-Index - 156
eISSN - 1365-2427
pISSN - 0046-5070
DOI - 10.1111/j.1365-2427.2004.01270.x
Subject(s) - hypolimnion , chlorophyll a , phytoplankton , photosynthesis , water column , biology , chlorophyll , botany , photosynthetically active radiation , microbial population biology , bacteria , environmental science , ecology , nutrient , genetics , eutrophication
Summary 1. Fine‐scale physical and chemical gradients and deep photosynthetic microbial populations were assessed to provide an initial characterisation of a small, thermally stratified reservoir (Cross Reservoir, Kansas, U.S.A.) and its deep chlorophyll maxima (DCM). Factors were identified that may affect vertical positioning of subepilimnetic photosynthetic sulphur bacteria (PSB) in lakes. 2. Results indicate that Cross Reservoir is a mesotrophic, dimictic lake with large subepilimnetic chlorophyll maxima containing dense layers of PSB. Characteristics of the deep PSB community of Cross Reservoir strongly correlate with both light and nutrient gradients. 3. The deep bacterial community mostly contained single‐celled and aggregating green sulphur bacteria, specifically free‐living Chlorobium limicola and the conspicuous motile ectosymbiotic consortium known as ‘ Chlorochromatium aggregatum ’. The bacteria were within the anaerobic hypolimnion, beneath a metalimnetic plate of Cryptomonas spp. and within very low sulphide and light conditions [mean of 67 μ gS L −1 and 0.05% photosynthetically active radiation (PAR)]. Pigment concentrations and fluorescence trends indicate that the bacteria made up a larger proportion of the DCM biomass than did phytoplankton in 1996. 4. Cross Reservoir shares characteristics with natural lakes world‐wide that also include a deep PSB community containing dense layers of ‘ C. aggregatum ’. Correlation analyses indicate that PSB community positioning and density are related to light, sulphide supply, redox potentials and pH. A 2‐factor principal components analysis (PCA) and other data trends supported these interpretations and indicated that PSB are sensitive to the thermal stability of the water column, are nitrogen limited and regulated more by sulphide or sulphide to light ratios than local levels of light. The sensitivity of these deep photosynthetic bacteria to environmental gradients, and their significance to some aquatic systems, demonstrate their potential as indicators of environmental disturbance.