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Adenylate energy charge in streambed sediments
Author(s) -
KAPLAN LOUISA,
BOTT THOMAS L.
Publication year - 1985
Publication title -
freshwater biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.297
H-Index - 156
eISSN - 1365-2427
pISSN - 0046-5070
DOI - 10.1111/j.1365-2427.1985.tb00187.x
Subject(s) - energy charge , adenylate kinase , environmental chemistry , algae , biology , respiration , monoculture , zoology , ecology , chemistry , botany , biochemistry , receptor
SUMMARY.1 The adenylate energy charge (EC A ) of microbial communities from streambed sediments was measured during three different seasons, under experimental manipulation and in culture. 2 The EC A values of sediments ( x ±S.E.) in the autumn, winter and spring were low and constant; 0.22±0.03 ( n =12), 0.32±0.04 ( n = 12) and 0.28±0.03 ( n =6) respectively. 3 A 5 h exposure of sediments to an algal lysate at 3.0–4.0°C and a 48 h exposure of sediments to tryptone‐yeast extract at 8.0–18.0°C failed to increase EC A even though respiration increased 3.7‐fold during the latter exposure. 4 The cellular EC A of a bacterial monoculture, sampled in log phase, was 0.90±0.10 ( n =3), but cxtracclluhir AMP depressed the total culture EC A to 0.21±0.01 ( n =4). 5 Attempts to isolate extracellular AMP from the interstitial waters of sediments were unsuccessful. 6 The data suggest that under natural conditions EC A is of limited use as a monitor of subtle changes in the physiological state of microbial communities in streambed sediments.