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Methods for the isolation, sterilization and cultivation of Lemnaceae
Author(s) -
BOWKER DAVID W.,
DUFFIELD ANTHONY N.,
DENNY ṔARICK
Publication year - 1980
Publication title -
freshwater biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.297
H-Index - 156
eISSN - 1365-2427
pISSN - 0046-5070
DOI - 10.1111/j.1365-2427.1980.tb01212.x
Subject(s) - frond , sodium hypochlorite , biology , sterilization (economics) , algae , isolation (microbiology) , botany , microbiology and biotechnology , chemistry , organic chemistry , monetary economics , economics , foreign exchange market , foreign exchange
SUMMARY. A method is described for isolating and sterilizing fronds of Lemnà gibba, L. minor, L. trisulca, L. polyrrhiza and Wolffia arrhiza , employing enrichment followed by sodium hypochlorite treatment. The method enables about 4% of the total number of treated fronds to be used as initiators of clonal cultures in which bacteria responding to organic enrichment and algae are excluded. Appropriate growth media, culture vessels and culture conditions are detailed.