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Increased sensitivity in the measurement of ATP in freshwater samples with a comment on the adverse effect of membrane filtration
Author(s) -
JONES J. G.,
SIMON B. M.
Publication year - 1977
Publication title -
freshwater biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.297
H-Index - 156
eISSN - 1365-2427
pISSN - 0046-5070
DOI - 10.1111/j.1365-2427.1977.tb01673.x
Subject(s) - lysis , pipette , chromatography , membrane , dilution , filtration (mathematics) , chemistry , boiling , biochemistry , physics , statistics , mathematics , organic chemistry , thermodynamics
SUMMARY. Increased sensitivity in the determination of ATP in fresh water and sediment samples was achieved by incorporating protein, EDTA and extra luciferin in the reconstruction of the firefly lantern preparation. Maximum recovery was only obtained when samples were extracted in actively boiling Tris buffer. The method was sufficiently sensitive for the determination of ATP in 1 ml water samples injected directly into the boiling buffer. Concentration of water samples on membrane filters, even volumes as small as 1 ml, caused a significant drop in the ATP recovered. This was thought to be due, at least in part, to cell lysis and the concentration of interfering detrital material on the membrane. Further interference was observed when sediment samples were analysed. This was only partly overcome by dilution of the sample and the importance of the inclusion of internal standards (bacterial cultures) is emphasized.