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A NEW METHOD FOR ESTIMATING THE PHOSPHOLIPID CONTENT OF SOILS
Author(s) -
BAKER R. T.
Publication year - 1975
Publication title -
journal of soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.244
H-Index - 111
eISSN - 1365-2389
pISSN - 0022-4588
DOI - 10.1111/j.1365-2389.1975.tb01967.x
Subject(s) - chloroform , soil water , extraction (chemistry) , benzene , methanol , chromatography , phospholipid , chemistry , volume (thermodynamics) , solvent , ethanol , environmental science , organic chemistry , soil science , biochemistry , membrane , thermodynamics , physics
Summary A simple and effective procedure for estimating soil phospholipids is described. The method uses Soxhlet extraction with (i) ethanol plus benzene (1:4 by volume) followed by (ii) methanol plus chloroform (1:1 by volume). The method compared favourably with the standard procedure of Hance and Anderson (1963) with the advantage that no acid pre‐treatment of the soil was required.