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GEL CHROMATOGRAPHY OF HUMIC ACID
Author(s) -
SWIFT R. S.,
POSNER A. M.
Publication year - 1971
Publication title -
journal of soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.244
H-Index - 111
eISSN - 1365-2389
pISSN - 0022-4588
DOI - 10.1111/j.1365-2389.1971.tb01610.x
Subject(s) - sephadex , chemistry , humic acid , chromatography , gel permeation chromatography , fractionation , size exclusion chromatography , adsorption , elution , silica gel , organic chemistry , fertilizer , enzyme , polymer
Summary Gel‐solute interactions have been reported in the application of gel chromatography to humic acid. The origins and nature of these interactions are discussed with particular reference to the behaviour of ‘Sephadex’ with compounds which are aromatic, heterocyclic, phenolic, or charged. Such structural units are known to be present in humic acid and could account for its observed interactions with ‘Sephadex’gel. The behaviour of humic acid on ‘Sephadex’and a number of other gel materials in a variety of eluants is examined. The observed deviations of these systems from that of an ideal gel chromatography system are explained in terms of charge and adsorption effects. The results indicate that a fractionation based solely on molecular weight differences can be achieved by using an alkaline buffer containing a large ammo cation as eluant together with ‘Bio‐gel’, ‘Sephadex’ or an agar based gel. Systems in which gel‐solute interactions occur should not be used for molecular weight fractionations.