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THE ISOLATION OF NUCLEOSIDE DIPHOSPHATES FROM ALKALINE EXTRACTS OF SOIL
Author(s) -
ANDERSON G.
Publication year - 1970
Publication title -
journal of soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.244
H-Index - 111
eISSN - 1365-2389
pISSN - 0022-4588
DOI - 10.1111/j.1365-2389.1970.tb01156.x
Subject(s) - chemistry , alkaline hydrolysis , pyrimidine , thymine , nucleoside , uracil , hydrolysis , ion chromatography , sodium hydroxide , nucleotide , chromatography , paper chromatography , dna , thymidine , biochemistry , organic chemistry , gene
Summary Two pyrimidine nucleoside diphosphates have been isolated from sodium hydroxide extracts of soils. They were initially detected in fractions which had been separated by ion‐exchange chromatography, and were purified by a combination of ion‐exchange and paper chromatography. Their ultraviolet spectra indicated that one ester contained thymine and the other uracil, and this was confirmed by hydrolysis. The ratio of P to pyrimidine was 2, but their acid stability showed that the esters were not nucleoside‐5’ pyrophosphates. Both gave a positive reaction to a colour test for deoxypentose and comparison with reference samples prepared from calf‐thymus DNA showed that they were thymidine‐3′: 5’ diphosphate and deoxyuridine‐3′:5’ diphosphate. The nucleoside diphosphates may be artefacts, derived from polynucleotides by acid and alkaline hydrolysis, but their isolation confirms, for the first time, the existence in soil of characteristic structural units of DNA.