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ORGANIC PHOSPHATE COMPOUNDS IN CALCIUM CHLORIDE EXTRACTS OF SOILS: IDENTIFICATION AND AVAILABILITY TO PLANTS
Author(s) -
WILD A.,
OKE O. L.
Publication year - 1966
Publication title -
journal of soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.244
H-Index - 111
eISSN - 1365-2389
pISSN - 0022-4588
DOI - 10.1111/j.1365-2389.1966.tb01479.x
Subject(s) - phosphate , chemistry , hydrolysis , inositol phosphate , chromatography , inositol , formic acid , chloride , ion chromatography , elution , calcium , ion exchange , phosphorus , biochemistry , organic chemistry , ion , receptor
Summary The organic phosphate in CaCl 2 extracts of dried soils was concentrated and purified by the use of ion exchange resins. Graded elution with formic acid separated three phosphate fractions (P1, P2, and P3), all of which contained inositol. By paper chromatography and assay of inositol, the phosphate in two of these fractions (P2 and P3) was found to be present mainly as myo‐inositol monophosphate. One form (P2) was resistant to hydrolysis and had a low availability to plants grown under aseptic conditions. The other (P3) was not pure, but was more easily hydrolysed and was readily used by plants. The phosphate in the remaining fraction (P1) was also available to plants and may also have been present as myo‐inositol monophosphate.