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Predictors of indeterminate IFN‐γ release assay in screening for latent TB in inflammatory bowel diseases
Author(s) -
Papay Pavol,
Eser Alexander,
Winkler Stefan,
Frantal Sophie,
Primas Christian,
Miehsler Wolfgang,
Angelberger Sieglinde,
Novacek Gottfried,
Mikulits Andrea,
Vogelsang Harald,
Reinisch Walter
Publication year - 2011
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.2011.02502.x
Subject(s) - indeterminate , inflammatory bowel diseases , medicine , inflammatory bowel disease , latent tuberculosis , immunology , pathology , tuberculosis , disease , mycobacterium tuberculosis , mathematics , pure mathematics
Eur J Clin Invest 2011; 41 (10): 1071–1076 Abstract Background IFN‐γ release assays (IGRA), widely used for latent tuberculosis screening prior to anti‐TNF‐α treatment, are limited by indeterminate results in patients under immunomodulatory (IM) therapy. The aim of our observational study was to delineate factors associated with indeterminate IGRA results. Methods A total of 190 patients with inflammatory bowel disease were included. IGRA was indeterminate if the result of IFN‐γ concentration was < 0·35 IU mL −1 for tuberculosis‐specific antigens and < 0·5 IU mL −1 for the positive control. Predictors for indeterminate results were delineated from multivariate logistic regression. Results IFN‐γ release assays was indeterminate in 26/190 (13·7%) patients. Indeterminate IGRA were associated with lower serum albumin levels (odds ratio [OR] 0·88, 95% confidence interval [CI] 0·79–0·96), lower absolute lymphocyte count (OR 0·39, 95% CI 0·18–0·75) and double IM therapy (OR 2·98, 95% CI 0·95–8·90). Sub‐analysis of IM therapy revealed an association of steroid therapy with indeterminate IGRA (OR 3·19, 95% CI 1·35–7·70). Hypoalbuminaemia increased the risk of indeterminate IGRA by (OR 2·97, 95% CI 1·03–8·61) and lymphopaenia by (OR 3·28, 95% CI 1·41–7·65). After a mean of 18·5 ± 14·4 days, retesting of IGRA in 18 patients with indeterminate results yielded 9 negative vs. 9 indeterminate results. Conclusions Our results reveal associations of indeterminate IGRA with low serum albumin levels and absolute lymphocyte count and double IM therapy. IGRA testing appears best to be performed prior to initiation of IM therapy in patients with inflammatory bowel disease.