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Parenteral lipids impair pneumococcal elimination by human neutrophils
Author(s) -
Versleijen Michelle W.,
Roelofs Hennie M.,
Te Morsche Rene H.,
Simonetti Elles R.,
Hermans Peter W.,
Wanten Geert J.
Publication year - 2010
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.2010.02320.x
Subject(s) - antibody opsonization , phagocytosis , streptococcus pneumoniae , phagocyte , microbiology and biotechnology , biology , mononuclear phagocyte system , streptococcaceae , immunology , bacteria , bacterial pneumonia , neutrophile , in vitro , biochemistry , antibiotics , opsonin , genetics
Eur J Clin Invest 2010; 40 (8): 729–734 Abstract Background Lipid‐induced modulation of phagocyte function seems to contribute to increased susceptibility to infections in patients on parenteral nutrition, and an increased risk for development of pneumonia has been observed in this group. The role of various structurally different lipid emulsions, however, remains unclear. In this study, we therefore assessed phagocyte function, as the capacity of neutrophils to eliminate Streptococcus pneumoniae (i.e. combined result of phagocytosis and killing), in the presence of these lipids. Materials and methods Neutrophils from six healthy volunteers were incubated for 1 h in emulsions (5 mmol L −1 ) derived from soybean‐ (LCT), fish‐ (VLCT), olive‐ (LCT‐MUFA), mixed soybean/coconut oils (LCT/MCTs) or structured lipids (SL). After opsonization of the pneumococci (strain OREP‐4) by human immunoglobulins, bacteria and neutrophils were incubated in the presence of complement. Next, pneumococcal elimination was evaluated and expressed as the percentage of bacteria eliminated relative to the initial bacterial numbers in neutrophil‐free samples. Results Neutrophils that were not exposed to lipids showed a pneumococcal elimination capacity of 75 ± 3% (mean ± SD). This significantly decreased after exposure to LCT‐MUFA (70 ± 6%), VLCT (67 ± 2%), SL (63 ± 9%), LCT (66 ± 10%) and LCT/MCT (47 ± 15%). Conclusion These data demonstrate that parenteral lipids impair the microbial elimination capacity of neutrophils in a structure‐dependent manner. In accordance with our previously reported in vitro effect on a range of phagocyte functions, LCT/MCT is by far the most potent in this respect.