Premium
Expression of inflammatory molecules and associations with BMI in children
Author(s) -
Dedoussis George V. Z.,
Kapiri Alexandra,
Samara Anastasia,
Dimitriadis Dimitris,
Lambert Daniel,
Pfister Michele,
Siest Gerard,
VisvikisSiest Sophie
Publication year - 2010
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.2010.02277.x
Subject(s) - adipokine , leptin , adipose tissue , medicine , endocrinology , peripheral blood mononuclear cell , body mass index , gene expression , tumor necrosis factor alpha , obesity , biology , gene , genetics , in vitro
Eur J Clin Invest 2010; 40 (5): 388–392 Abstract Background Adipose tissue secrets several adipokines that have been proposed to be enrolled in many inflammatory pathways. Our aim was to investigate the adipokine expression in adipose tissue and peripheral blood mononuclear cells (PBMCs) in children. Materials and methods Thirty‐one (17 males and 14 females) healthy children aged 10·9 ± 1·8 years with a body mass index (BMI) of 19·3 ± 3·5 kg m −2 were enrolled. Adipokines (TNF‐alpha, IL‐6 and leptin) gene expression was quantified by real‐time quantitative PCR in adipose tissue and PBMCs from the same children. Their serum levels were also measured. Results BMI was positively correlated with leptin gene expression in adipose tissue and with leptin serum levels (β = 0·476, P = 0·006 and β = 0·576, P = 0·003 respectively). Leptin’s serum levels were positively correlated with leptin gene expression in adipose tissue (β = 0·462, P = 0·02). Adipose tissue gene expression of leptin and TNF‐alpha and serum leptin and TNF‐alpha serum levels were positively correlated (β = 0·752, P < 0·001, β = 0·311 and P = 0·015 respectively). In PBMCs, a positive correlation between TNF‐alpha and IL‐6 expression was found (β = 0·526, P = 0·042). Conclusion We demonstrated powerful correlations of adipokines gene expression in adipose tissue and PBMCs in children, underlying that these molecules share common pathways related to childhood obesity.