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Choline supplementation reduces oxidative stress in mouse model of allergic airway disease
Author(s) -
Mehta A. K.,
Arora N.,
Gaur S. N.,
Singh B. P.
Publication year - 2009
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.2009.02190.x
Subject(s) - oxidative stress , ovalbumin , lipoic acid , lipid peroxidation , chemistry , bronchoalveolar lavage , antioxidant , endocrinology , reactive oxygen species , medicine , pharmacology , immunology , biochemistry , lung , immune system
Background Asthma is a multi‐factorial inflammatory disease associated with increased oxidative stress and altered antioxidant defences. We have evaluated the effect of choline on oxidative stress in a mouse model of airway disease. Materials and methods Balb/c mice were sensitised with 100 μg of ovalbumin on days 0 and 14, and challenged with aerosolized ovalbumin on days 25–27. Mice were administered 1 mg kg –1 of choline via oral gavage or intranasal route on days 14–27. Mice were also administered 100 mg kg –1 of α‐lipoic acid as standard antioxidant. Total cell counts, eosinophils and eosinophil peroxidase (EPO) activity were determined in bronchoalveolar lavage (BAL) fluid. Reactive oxygen species (ROS), lipid peroxidation and isoprostanes levels were measured in BAL fluid. IL‐13 and tumour necrosis factor‐alpha (TNF‐α) levels were also measured in BAL fluid and spleen cell culture supernatant. Nuclear factor κB (NFκB) p65 protein expression was measured after last ovalbumin challenge in nuclear and cytosolic extracts of lungs. Results Compared with ovalbumin‐challenged mice, choline and α‐lipoic acid treated mice had significantly reduced eosinophilic infiltration and EPO activity in BAL fluid. Choline and α‐lipoic acid treatment reduced ROS production and isoprostanes level significantly in BAL fluid and thus suppressed oxidative stress. Choline and α‐lipoic acid administration by either route decreased lipid peroxidation levels and down regulated NFκB activity. Further, choline and/or α‐lipoic acid treatment suppressed TNF‐α level significantly as compared with that of ovalbumin‐challenged mice. Conclusions Choline administration reduces oxidative stress possibly by modulating the redox status of the cell and inhibits inflammatory response in a mouse model.