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Changes in free radical generation and antioxidant capacity during ultramarathon foot race
Author(s) -
Skenderi K. P.,
Tsironi M.,
Lazaropoulou C.,
Anastasiou C. A.,
Matalas AL.,
Kanavaki I.,
Thalmann M.,
Goussetis E.,
Papassotiriou I.,
Chrousos G. P.
Publication year - 2008
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.2007.01917.x
Subject(s) - oxidative stress , antioxidant capacity , antioxidant , reactive oxygen species , medicine , endocrinology , glutathione , chemistry , biochemistry , enzyme
Background Exhaustive exercise has been implicated in the generation of reactive oxygen species, resulting in oxidative stress. We studied the effect of a long‐distance, endurance exercise on oxidative stress parameters in athletes who participated in the ultramarathon race Spartathlon (246 km). Materials and methods This study included 18 runners (16 men and 2 women) aged 42·8 ± 1·4 years. Blood samples were obtained 24 h before (prerace), at the end (postrace) and 48 h after the end of the race (48 h postrace). We measured oxidative stress indices, including red cell glutathione, malonyldialdehyde and 8‐iso‐prostaglandin F 2a , as well as the total antioxidant capacity. Results 8‐Iso‐prostaglandin F 2a level increased significantly at the end of the race, compared to prerace levels (up to 914·7 ± 61·4 pg mL −1 from 197·6 ± 8·4 pg mL −1 ), and remained 2·5‐fold increased over the baseline 48 h after the race (532·0 ± 54·2 pg mL −1 , P < 0·000). The total antioxidant capacity of the athletes increased from a baseline of 289·6 ± 9·0 µmol L −1 to 358·7 ± 11·0 µmol L −1 immediately after the race and remained elevated 48 h later (350·6 ± 7·6 µmol L −1 ) ( P < 0·001). Conclusions Prolonged exercise induces a marked response of oxidative stress biomarkers, which in part is compensated by serum ability to scavenge free radicals. Whether these changes have long‐term negative effects in the organism needs further investigation.