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Acute lung injury following pancreas ischaemia‐reperfusion: role of xanthine oxidase
Author(s) -
Flaishon R.,
Szold O.,
Weinbroum A. A.
Publication year - 2006
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.2006.01723.x
Subject(s) - xanthine oxidase , bronchoalveolar lavage , medicine , pancreatitis , lung , acute pancreatitis , pancreas , endocrinology , chemistry , pathology , anesthesia , biochemistry , enzyme
Background Acute pancreatitis can lead to increased pulmonary vascular permeability and respiratory failure. Oxidants (and their generator, xanthine oxidase (XO)) play an important role in injuring the structural integrity of the pulmonary epithelium and endothelium, but their importance in the induction of acute lung injury following pancreas ischaemia‐reperfusion (IR) has not been defined. Materials and methods Rats ( n = 48) received a regular or a tungsten (oxidoreductase inhibitor)‐enriched diet for 14 days. Their isolated pancreases were then either perfused (controls) or made ischaemic (IR) for 40 min (12 replicates/group). This was followed by in‐series pancreas plus normal isolated lung reperfusion for 15 min. Lungs only were subsequently perfused with the 15‐min accumulated pancreas effluents for 45 min. Results Injury was induced in all IR pancreases as expressed by reperfusion pressure, wet‐to‐dry ratio and amylase and lipase concentrations. Tissue XO activity was high and reduced glutathione pool was low in the tungsten‐free IR pancreases. Pulmonary plateau pressure increased by 46% and final PO 2 /FiO 2 decreased by 24%. Capillary pressure and weight rose two‐ to fourfold in lungs paired with IR non‐treated pancreases. Twofold increases in bronchoalveolar lavage volume and contents, including XO, were also recorded in this group of lungs. Lungs exposed to tungsten‐treated ischaemic pancreas effluents were minimally damaged and tissue XO content was low compared to controls. Conclusions Ex‐vivo acute pancreatitis induces acute lung injury via oxidants/antioxidants misbalance, which may be prevented by attenuating pancreas oxidative stress.