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Lipoprotein profiles in plasma and interstitial fluid analyzed with an automated gel‐filtration system
Author(s) -
Parini P.,
Johansson L.,
Bröijersén A.,
Angelin B.,
Rudling M.
Publication year - 2006
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.2006.01597.x
Subject(s) - very low density lipoprotein , lipoprotein , chromatography , chemistry , centrifugation , cholesterol , triglyceride , high density lipoprotein , size exclusion chromatography , interstitial fluid , low density lipoprotein , medicine , biochemistry , enzyme
Background High‐quality methods for lipoprotein characterization are warranted in studies on various metabolic diseases. Materials and methods An automated system for size‐exclusion chromatography (SEC) of lipoproteins using commercially available components is described. Cholesterol or triglyceride content in separated lipoproteins from plasma and interstitial fluid (IF) was continuously determined on‐line using microlitre sample volumes. Results The lipoprotein assay showed a good concordance with the classic ultra‐centrifugation/precipitation technique using fresh or frozen samples. Determination of lipoproteins in IF obtained from vacuum‐induced skin blisters from 18 healthy subjects revealed that very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoprotein (HDL) cholesterol levels were 18%, 19% and 25%, respectively, of concomitant plasma concentrations. The size‐exclusion chromatography (SEC) system also allows for triglyceride determination on‐line and it could be shown that the system is advantageous for an accurate determination of triglycerides in conditions when there are high levels of glycerol, e.g. in mice and in patients with hyperglycerolaemia (pseudo‐hypertriglyceridaemia). Conclusions The described system should be of value in studies where detailed lipoprotein analysis is warranted and particularly when significant sample series with small volumes are available. Our data also suggest that there is a 4–5·5‐fold concentration gradient between plasma and IF for the three major plasma lipoproteins.