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Identification of different bacterial DNAs in human coronary arteries
Author(s) -
Lehtiniemi J.,
Karhunen P. J.,
Goebeler S.,
Nikkari S.,
Nikkari S. T.
Publication year - 2005
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.2005.01440.x
Subject(s) - coronary arteries , autopsy , coronary atherosclerosis , coronary artery disease , sudden death , biology , cardiology , polymerase chain reaction , artery , bacteria , medicine , pathology , microbiology and biotechnology , genetics , gene
Background Various studies have suggested a link between infection, atherosclerosis and coronary artery disease. We studied whether bacterial DNA is present in coronary specimens obtained from left anterior descending coronary arteries of subjects having sudden deaths of cardiovascular and other causes, as verified by an autopsy. Materials and methods Coronary specimens were obtained from five subjects who died of sudden coronary causes and five controls. Broad‐range 16‐s rDNA PCR (Br‐PCR) amplification, cloning and sequencing were used to detect bacterial rDNA. Results Bacterial rDNA sequences of oral pathogens were detected from the coronary samples in all cases regardless of the cause of death. Conclusions Br‐PCR is a powerful method to detect bacterial rDNA. By this method we were able to detect wide palette of oral bacteria from coronary tissues. Our findings suggest that atheromas may act as mechanical sieves collecting bacteria from the circulation.