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Transforming growth factor β 1 modulates angiotensin induced calcium influx in vascular smooth muscle
Author(s) -
ZHU Z.,
TEPEL M.,
NEUSSER M.,
ZIDEK W.
Publication year - 1995
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1995.tb01708.x
Subject(s) - medicine , angiotensin ii , endocrinology , vascular smooth muscle , calcium , chemistry , fura 2 , calphostin c , cytosol , protein kinase c , transforming growth factor beta , transforming growth factor , biology , kinase , smooth muscle , biochemistry , blood pressure , enzyme
. The modulatory effects of transforming growth factor β 1 (TGF β 1 ) on the angiotensin II (Ang II)‐induced increase in cytosolic free calcium concentration ([Ca 2+ ] i ) were investigated in vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) and normotensive Wistar‐Kyoto rats (WKY). [Ca 2+ ] i in VSMC was measured using the fluorescent dye fura‐2. When TGF β 1 was applied 30 s prior to Ang II, the Ang II‐induced [Ca 2+ ] i increase was significantly enhanced in VSMC from SHR ( P < 0.05 compared to control), whereas after the preincubation with TGF β 1 for 30 min, the Ang II‐induced [Ca 2+ ] i increase was significantly reduced in VSMC from both strains. Using the manganese‐quenching technique, it was confirmed that short‐term exposure to TGF β 1 enhanced the Ang II‐induced trans‐plasma‐membrane calcium influx in SHR. The inhibition of protein kinase C by calphostin C abolished the stimulatory effect of TGF β 1 on the Ang II‐induced [Ca 2+ ] i increase. It is concluded that TGF β 1 modulates the Ang II‐induced calcium handling in VSMC.