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Lack of plasma lipid peroxidation during LDL‐apheresis by heparin‐induced extracorporeal LDL‐precipitation
Author(s) -
WIELAND E.,
SCHETTLER V.,
CREUTZFELDT C.,
KICKBUSCH H.,
SCHUFFWERNER P.
Publication year - 1995
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1995.tb01693.x
Subject(s) - tbars , ldl apheresis , lipid peroxidation , extracorporeal , extracorporeal circulation , chemistry , apheresis , medicine , heparin , cholesterol , thiobarbituric acid , endocrinology , lipoprotein , oxidative stress , platelet
. The heparin‐induced extracorporeal precipitation of low density lipoproteins (HELP) is a well established clinical apheresis procedure to markedly reduce cholesterol levels. The biocompatibility of this filter system was investigated by the determination of lipid peroxidation products. Both lipid hydro‐peroxides and thiobarbituric acid reactive substances (TBARS) were determined before, during and after 38 aphereses in 21 patients undergoing regular HELP treatment. Although HELP patients had significantly elevated TBARS compared to 93 healthy controls (3.13 ±0.64 vs. l.66±0.50 μmol L ‐1 ; P <0.01), no significant differences were observed compared to either 104 patients suffering from angiographically confirmed coronary heart disease (3.42 ±0.81 μmol L ‐1 ; P > 0.05 vs. HELP patients) or 38 aged‐matched hyperlipidaemic patients (3.30 ±0.75 μmol L ‐1 ; P >0.05 vs. HELP patients), neither of which were included in the HELP programme. No lipid hydroperoxides were detected in the plasma of HELP patients either before or after the extracorporeal treatment. After the LDL‐apheresis TBARS were significantly decreased (2.60 ± 0.52 μmol L ‐1 ) compared to the values before the treatment ( P <0.01). There was no evidence for the formation of lipid hydroperoxides within the HELP circuit. It is suggested, therefore, that plasma lipids are not oxidized by the HELP procedure.